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. Author manuscript; available in PMC: 2018 Sep 15.
Published in final edited form as: Cancer Res. 2017 Jul 28;77(18):5068–5076. doi: 10.1158/0008-5472.CAN-17-0567

Figure 4. Synergistic toxic effect of AZD7762 and gemcitabine on Lkb1-null cells.

Figure 4

Lkb1-null t2, t4, and t5 cells were treated with AZD7762 (0-500 nM) alone or in combination with gemcitabine at the indicated concentration for 4 days and then cell viability was determined using CellTiter-Glo Assays.

A, plot of relative cell viability (%) versus AZD7762 at gradient constant and gemcitabine (Gemc) at constant concentration. Significance of AZD7762 alone versus combined AZD7762 and gemcitabine was calculated by two-way ANOVA. ****, p value ≤ 0.0001.

B, bar graph showing IC50 of AZD7762 alone or in combination with gemcitabine (Gemc) on Lkb1-null cell lines as calculated by GraphPad.

C, CI values calculated using CalcuSyn software. A CI of 0.1-0.3 indicates a strong synergy effect, and a CI of 0.3-0.7 indicates a synergy effect.