Table 1.

Islet donor characteristics and specific autoreactivity of islet-derived T cells

Organ donor ID	Age (years)	Sex	T1D duration (years)	Donor HLA	T cell	Autoantigen	HLA restriction	Reference
Donor A	19	M	3	A1, A2	8 CD4+ T cell clones	Proinsulin42–50	DQ8	[74••]
				B8, B51	1 CD4+ T cell clone	Proinsulin41–51	DQ8	[74••]
				DR3, DR4	1 CD4+ T cell clone	Proinsulin41–49	DQ8	[74••]
				DQ2, DQ8	1 CD4+ T cell clone	Proinsulin50–59	DQ8	[74••]
					1 CD4+ T cell clone	Proinsulin50–58	DQ8	[74••]
					2 CD4+ T cell clones	Proinsulin52–62	DQ8transa	[74••]
					1 CD4+ T cell clone	HIP C-peptide: IAPP2b	DQ8	[75••]
					1 CD4+ T cell clones	HIP C-peptide: IAPP2b	DQ8	[75••]
nPOD6342	14	F	2	A2, A68	1 CD4+ T-cell clone	GAD274–286	DR4c	[76••]
				DR1, DR4 DQ5, DQ8	1 CD4+ Transductant (GSE.20D11)d	Insulin B9–23	DQ8	[77••]
nPOD6367	24	M	2	A2, A29 DR4, DR7 DQ2, DQ8	1 CD4+ T cell line	HIP C-peptide:A-chainb	ND	[76••]
nPOD6268	12	F	3	A2, A68 DR17, DR13 DQ2, DQ6	3 CD8+ T cell lines	Pooled insulin B10–18, IA-2797–805, IGRP265–273 tetramers	A*02:01	[76••]
nPOD69	6	F	3	A2, A26	1 CD4+ T cell line	Proinsulin76–90e	DR4c	[76••]
				DR4, DR7	1 CD4+ T cell line	Chromogranin Af	ND	[76••]
				DQ2, DQ8	2 CD4+ T cell lines	Proinsulinf	ND	[76••]
nPOD6323	22	F	6	A1, A25	1 CD4+ T cell line	Chromogranin Af	ND	[76••]
				DR4, DR17	1 CD4+ T cell line	HIP C-peptide:IAPP1b	ND	[76••]
				DQ2, DQ8	1 CD4+ T cell line	HIP C-peptide: IAPP2b	ND	[76••]
					1 CD4+ T cell line	GRP78292–305(Arg-Cit 297)	ND	[76••]
					1 CD4+ Transductant (GSE.8E3)d	Proinsulin49–65	DQ8transa	[77••]
					1 CD4+ Transductant (GSE.6H9)d	Insulin B9–23	DQ8	[77••]
T1D.6	20	M	7	A2, −	1 CD4+ T cell line	GAD555–567	DR4c	[76••]
				DR17, DR4 Q2, DQ8	1 CD4+ T cell line	HIP C-peptide:NP-Yb	DQ8	[75••, 76••]
T1D.7	27	M	17	A1, A3	1 CD4+ T cell clone	IAPP65–84(Arg-Cit 73, 81)	ND	[76••]
				DR17, DR4	1 CD4+ T cell clone	GAD115–127	ND	[76••]
				DQ2, DQ8	1 CD4+ T cell clone	IA-2545–562(Gln-Glu 548, 551, 556)	ND	[76••]

The age, gender, HLA, and duration of T1D of the islet donors are shown along with the original citations. In this on-going project, these are the autoreactivities of islet-infiltrating T cell lines or clones identified, to date, and the donors from which the islet-infiltrating T cells were derived. (Adapted from: Babon JA, et al. Nat Med. 2016;22:1482–1487) [76••]

ND not determined

^aHLA-DQ8trans: DQA1*05:01/DQB1*03:02

^bHIP hybrid insulin peptide: fusion of a human insulin C-peptide fragment (N-terminus ELGGG) with a fragment of another peptide (A-chain insulin A-chain fragment, IAPP1 and 2 two islet amyloid polypeptide fragments, NP-Y neuropeptide Y fragment)

^cHLA-DR4 were all HLA-DRB1*04:01

^dClonal CD4⁺ T-cell receptor transductant

^eProinsulin_76–90 (SLQPLALEGSLQKRG) is designated Proinsulin_52–66 by numbering starting with the B chain

^fEpitopes not identified