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. 2017 Sep 12;8:1110. doi: 10.3389/fimmu.2017.01110

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Copyright © 2017 Huang, Liu, Tang, Du, Feng, Hu, Zhu, Li and Suo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interferon-γ (IFN-γ) secretion by peripheral blood lymphocytes in the ELISPOT assay and enzyme-linked immunosorbent assay (ELISA). (A) Peripheral blood mononuclear cells (PBMCs, 5 × 10⁵ cells/well) from transgenic and wild-type pigs were stimulated with CD4⁺ or CD8⁺ epitopes of porcine reproductive and respiratory syndrome virus (PRRSV) for 24 h with concanavalin A (ConA) or bovine serum albumin-coated beads, acting as positive and negative control, respectively. Wells containing PBMC alone served as controls. Samples were tested in triplicates. Images from the plate scan generated by the ImmunoSpot analyzing system are shown. Average numbers of spots per well are indicated. (B) Summary of the spots per well in IFN-γ ELISPOT assay. (C) Results shown are the concentrations of IFN-γ in serum samples of wild-type (WT) and Tg pigs (ND, not detected). P < 0.05 was considered statistically significant.