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H&E staining analysis of OS samples at different stages. (A) The OS consists of an atypical round to cell proliferation with osteoid deposition at G1 stage (H&E stain ×200). (B) OS cells shape variable with chromatic nuclei and mitosis fields at G2 stages. (C) Spindle cell neoplasm has high cellularity, abnormal mitotic characters and atypical nuclear at G3 stage. (D) The OS forms a highly-cancerous and high-mortality bone tumor at G4 stage.
