Figure 4.

Migration speed of canine mammary tumor (CMT)-U27 and CMT-U309 on 2D environment. (A) Images from a wound-healing assay at different time points. CMT-U27 and CMT-U309 cells were grown to confluency, wounded with a pipette tip, and then imagined 24 h with automated cell IQ live-imaging platform. (B) Wound area recovery over time. The gap area was quantified for each time frame and for each cell line using Cell IQ Analyser. The half-closure times were calculated by using the formula: $t\text{1/2gap}=\frac{\text{Initial gap Area}}{\text{2xslope}}$. Mean ± SD is shown, n(U27) = 6 and n(U309) = 8. (C) Average migration speed (micrometers per hour) of CMT-U27 and CMT-U309 cell lines. Migration speed was calculated: $\upsilon \text{migration}=\frac{\text{slope}}{\text{2xl}}$. Values are presented in box-plots, where the median is indicated by the central bar. 5/95 percentile whiskers with outliers are shown; n(U27) = 6 and n(U309) = 8.