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. 2017 Sep 12;4:149. doi: 10.3389/fvets.2017.00149

Figure 5.

Figure 5

3D morphology and invasion potential of canine mammary tumor (CMT)-U27 and CMT-U309 cell lines. (A) Confocal images of CMT-U309 and CMT-U27 in 3D collagen matrix. CMT-U27 cells adopt round and blebbing conformation, while CMU309 cells exert long actin-based protrusions. Phalloidin-green, DAPI-pink. Scale bar 10 μm. (B) CMT cell lines have different invasion potential in 3D matrigel cultures. Brightfield images of cultures taken on days 1, 3, and 6 are shown. Both CMT-U27 and CMT-U309 cell lines form initially spheroid-like structures, which start to expand rapidly. CMT-U27 cells have deficient growth control and form very dense structures but do not invade, while CMT-U309 cells clearly start to dissociate from the original 3D spheroid and form secondary structures outside. Scale 100 μm. (C) Quantification of the diameter of the expanding cellular 3D structure 6 days after culture. Values are presented in box-plots, where the median is indicated by the central bar. 5/95 percentile whiskers with outliers are shown; n = 15.