Figure 2.

Behavior of CAI4-WOR1^OE cells at early stages of colonization and distribution along the gastrointestinal tract. (A) Fungal loads from each individual and mean ± SEM are represented at early stages as red squares (CAI4-RFP) or gray triangles (CAI4-WOR1^OE). (B) Comparison of percentages of CAI4-RFP and CAI4-WOR1^OE strains in the inoculum and in fecal samples at early time points (mean ± SEM). (C) Comparison of percentages of CAI4-RFP and CAI4-WOR1^OE strains in different parts of the gastrointestinal tract. (mean ± SEM). (D) Fitness of CAI4-WOR1^OE in co-housing experiments. Oral antibiotic therapy was given to mice (n = 3) from 4 days before a gavage of 10⁷ cells in a 1:1 mixture of CAI4-RFP (res squares) and CAI4-WOR1^OE (gray triangles) strains (day 0). On day 28, 2 antibiotic treated mice (open symbols) were placed together with already colonized mice. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.