Figure 4.

Role of WOR1 overexpression in oxidative metabolism. (A) Inhibition of the basal respiration in the presence of inhibitors of the electron transport chain. O₂ consumption was quantified by an oxygen electrode and expressed as the ratio of respiration in the presence of compounds vs. basal respiration rate for each analyzed strain. ^*p < 0.05. (B) 10⁵ cells and ten-fold dilutions from overnight growing cells in the presence or absence of doxycycline from the indicated strains were spotted onto plates supplemented with 0.02 ‰ sodium azide (Na Azide) and in the presence or absence of doxycycline (±Dox). (C) Cells from cultures of the CAI4-RFP, CAI4-WOR1^OE and WO-1 strains growing either at 20 or 37°C for 48 h were analyzed as in (B). Plates were incubated at 20 and 37°C and scanned at 96 and 24 h respectively in either normoxia or microaerophilia.