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. 2017 Sep 11;8:1695. doi: 10.3389/fmicb.2017.01695

FIGURE 2.

FIGURE 2

Susceptibilities of Francisella tularensis to AR-13 and AR-16. (A) LVS was grown in twofold serial dilutions of AR-13 and AR-16 in mTSB. Optical densities at 600nm (OD600) were measured by a plate reader 18 h after inoculation. (B) AR-13 has a bactericidal effect on LVS. Approximately 1.5 × 109 CFU were incubated in 1 ml PBS containing 10 μg of AR-13. Viable bacteria were evaluated at different time points by serial dilution and plating. (C) AR-16 has a bacteriostatic effect on LVS. Approximately 1.5 × 109 CFU were incubated in 1 ml PBS containing 10 μg of AR-16. Viable bacteria were evaluated at different time-points by serial dilution and plating. (D) AR-13 and AR-16 have bactericidal and bacteriostatic effects, respectively, on F. tularensis SchuS4. Approximately 1.5 × 109 CFU of F. tularensis SchuS4 was incubated in 1 ml PBS containing 10 μg of AR-13 or AR-16. Viable bacteria were evaluated at 8 h post-treatment by serial dilution and plating. Data are representative of 2–4 independent experiments, each performed in triplicate; ∗∗∗p < 0.001; NS, not significant.