Table 2.
Mass cytometry staining panel.
| Marker | Tag | Clone | Vendor |
|---|---|---|---|
| CD45 | 89Y | HI30 | Fluidigm |
| CD57 | 115In | HCD57 | BioLegend |
| CD196 (CCR6) | 141Pr | 11A9 | BD Pharmingen |
| CD19 | 142Nd | HIB19 | Fluidigm |
| CD5 | 143Nd | UCHT2 | Biolegend |
| CD195 (CCR5) | 144Nd | NP-6G4 | Fluidigm |
| CD4 | 145Nd | RPA-T4 | Fluidigm |
| CD8a | 146Nd | SK1 | BioLegend |
| CD11c | 147Sm | Bu15 | Fluidigm |
| CD31 | 148Nd | WM59 | BioLegend |
| CD278 (ICOS) | 151Eu | DX29 | Fluidigm |
| αβTCR | 152Sm | IP26 | BioLegend |
| CD3ε | 154Sm | UCHT1 | Fluidigm |
| CD194 (CCR4) | 155Gd | 205410 | R&D Systems |
| Vα2.3 | 156Gd | F1 | Thermo Scientific |
| CXCR3 | 157Gd | G025H7 | BioLegend |
| Vβ22 | 159Tb | IMMU 546 | Beckman Coulter Immunotech |
| CD28 | 160Gd | CD28.2 | BioLegend |
| CD161 | 161Dy | HP-3G10 | BioLegend |
| Ki-67 | 162Dy | B56 | Fluidigm |
| HLA-DR | 163Dy | L243 | BioLegend |
| CD44 | 164Dy | BJ18 | BioLegend |
| CD127 | 165Ho | A019D5 | Fluidigm |
| CD27 | 167Er | L128 | Fluidigm |
| CD38 | 168Er | HIT2 | BioLegend |
| CD45RA | 169Tm | HI100 | Fluidigm |
| CD152 (CTLA-4) | 170Er | 14D3 | Fluidigm |
| CD279 (PD-1) | 172Yb | EH12.2H7 | BioLegend |
| CD39 | 173Yb | A1 | BioLegend |
| CXCR5 | 174Yb | 51505 | R&D Systems |
| Cell-ID™ Intercalator-Ir (DNA) | 191Ir | – | Fluidigm |
| Cell-ID™ Intercalator-Ir (DNA) | 193Ir | – | Fluidigm |
| Cell-ID™ Cisplatin (Live–dead) | 195Pt | – | Fluidigm |
CD, cluster of differentiation; CTLA-4, cytotoxic T lymphocyte-associated protein 4; CCR, C–C chemokine receptor; ICOS, inducible co-stimulatory molecule; PD-1, programmed cell death protein 1; CXCR, CXC chemokine receptor; TCR, T cell receptor; HLA-DR, human leukocyte antigen-antigen D related; Sm, samarium; Nd, neodymium; Er, erbium; Gd, gadolinium; Yb, ytterbium; Dy, dysprosium; Y, yttrium; Tm, thulium; In, indium; Ho, holmium; Gd, gadolinium; Pr, praseodymium; Eu, europium; Pt, platinum; Ir, iridium.
Fluidigm, South San Francisco, CA, USA; BioLegend, San Diego, CA, USA; BD Pharmingen, San Diego, CA, USA; R&D Systems Inc., Minneapolis, MN, USA; Thermo Scientific, Rockford, IL. USA; Beckman Coulter Immunotech, Marseille, France.