FIGURE 3.

Effect of morin on apoptosis.SW480 cells were treated with various concentrations of morin (150, 200, and 250 μM) and camptothecin (50 μM) for 48 h. (A) Chromatin condensation and nuclear fragmentation observed using Hoechst 33342 staining, arrows represent cells with chromatin condensation inside the nucleus or fragmentation of the nucleus. (B) Annexin V-FITC/propidium iodide staining and flow cytometric analysis of cells undergoing apoptosis. (C) Flow cytometry analysis of the percentage of apoptotic cells in each group. CAPT: camptothecin (50 μM), M (150, 200, and 250 μM): Morin (150, 200, and 250 μM). Significance levels between different groups were determined by using one way ANOVA, followed by Duncan’s multiple range test. ^∗p ≤ 0.05 versus control, ^#p ≤ 0.05 versus camptothecin.