FIGURE 7.

Quantification of ROS formation by DCFH-DA staining. SW480 cells were treated with various concentrations of morin (150, 200, and 250 μM) for 24 and 48 h and H₂O₂ (200 μM) for 2 h. After incubation, cells were stained with DCFH-DA and analyzed using flow cytometer. (A) Representative results. (B) Data analyzing fluorescence intensity from triplicate measurements. (C) Fluorescent imaging using fluorescent microscope. M (150, 200, and 250 μM): Morin (150, 200, and 250 μM). Significance levels between different groups were determined by using one way ANOVA, followed by Duncan’s multiple range test. ^∗p ≤ 0.05 versus control.