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. 2017 Sep 12;8:1585. doi: 10.3389/fpls.2017.01585

FIGURE 1.

FIGURE 1

mRNA expression levels of barley C1A CysProt genes after 24 h of elicitor treatments. Expression of barley CysProt genes (HvPap-1, -4, -6, -12, -16 and -19) in leaves treated with (A) flagellin (Flg22) and (B) chitosan (Chit), controls (light colors) and treated (dark colors). Data were determined by RT-qPCR and expressed as mRNA levels of C1A CysProt genes normalized to barley cyclophilin mRNA content. One (∗) or two asterisks (∗∗) indicate significant differences between control and treatment as determined by a one-way ANOVA test (t-Student, P < 0.01 and P < 0.001, respectively). (C) Protein accumulation pattern of barley C1A CysProt (HvPap-1, -19, -6 and -16) after 24 h of elicitor treatments, assayed by immunoblot. Proteins were extracted from 7 days old barley leaves without treatment (control) and treated with flagellin (Flg22) or chitosan (Chit). Rubisco protein expression was analyzed using a specific antibody against its large subunit.