Skip to main content
. 2017 Sep 12;8:1585. doi: 10.3389/fpls.2017.01585

FIGURE 6.

FIGURE 6

Messenger expression levels of C1A CysProt genes (HvPap-1, -19, -12, -6, -4 and -16) in transgenic HvPap-1 overexpressing (OE Pap1, 919) and silencing (KD Pap1, 1175) lines, and wild-type (WT) barley plants during T. urticae infestation, assayed by RT-qPCR. (A) Total RNA was extracted from leaves after 14 days of infestation (dark red) and non-infested leaves (light red). Data were expressed as mRNA levels of C1A CysProt genes normalized to barley cyclophilin mRNA content. Different letters indicate significant differences (P < 0.01, one-way ANOVA Student Newman–Keuls test). (B) Protein accumulation patterns of C1A CysProt (HvPap-1, -19, -6 and -16) in transgenic (OE Pap1 and KD Pap1) and wild-type (WT) barley plants during T. urticae infestation assayed by immunoblot. Total protein was extracted form leaves after 14 d of infestation (I) and non-infested leaves (C). Rubisco protein expression was assayed using a specific antibody against its large subunit.