FIGURE 8.

Messenger expression levels of germin-like genes and protease inhibitors genes from I12 Bowman-Birk and I13 Potato Inhibitor I families, in transgenic HvPap-1 overexpressing (OE Pap1, 919) and silencing (KD Pap1, 1175) lines, and wild-type (WT) barley plants, assayed by RT-qPCR. Total RNA was extracted form leaves after (A) three (3d) or seven (7d) days of M. oryzae infection (light and dark green) or (B) seven (7d) or fourteen (14d) days of T. urticae infestation (light and dark red) and non-treated leaves (light gray). Data were expressed as mRNA levels normalized to barley cyclophilin mRNA content. Different letters indicate significant differences (P < 0.01, one-way ANOVA Student Newman–Keuls test).