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. 2017 Sep 12;11:282. doi: 10.3389/fncel.2017.00282

Figure 2.

Figure 2

Characterization of mitochondria (Mch) and mitochondria-depleted brain fraction (Brain (-Mch)) (A) and subunit composition of nicotinic acetylcholine receptors (nAChRs) in the whole brain, mitochondria or mitochondria-depleted brain fraction of WT and α9−/− mice (B). (A) The samples were studied by Sandwich ELISA using the antibodies against voltage-dependent anion channel (VDAC), lamin B1 or inositol-requiring enzyme-1α (IRE-1α). (B) The samples were captured with α7(1–208)-specific antibody and revealed with α3(181–192)-, α4(181–192), α5(180–191), α7(179–190)-, α9(190–200)-, α10(404–417)-, β2(190–200) or β4(190–200)-specific antibodies. The columns correspond to M ± SE; **p < 0.005, ***p < 0.0005 compared to corresponding OD values of WT mice, n = 5.