Table 1.
Yeast expression plasmids and yeast strains used in this work.
| Strain | Transformed plasmids | Expressed proteins | Full-length (kDa) | Processed (kDa) |
|---|---|---|---|---|
| SB09Y | pN2GLT4 | SU9-NifU | 40.6 | 33.6 |
| SU9-NifS | 51.0 | 44.0 | ||
| pN2SB22 | SU9-FdxN | 17.1 | 9.8 | |
| SU9-NifBAv-His10 | 63.6 | 56.3 | ||
| SB10Y | pN2GLT4 | SU9-NifU | 40.6 | 33.6 |
| SU9-NifS | 51.0 | 44.0 | ||
| pN2SB24 | SU9-FdxN | 17.1 | 9.8 | |
| SU9-NifBMi-His10 | 43.8 | 36.4 | ||
| SB03Y | pN2SB15 | SU9-FdxN | 17.1 | 9.8 |
| SU9-His10-NifBAv | 63.6 | 56.3 | ||
| SB12Y | pN2GLT4 | SU9-NifU | 40.6 | 33.6 |
| SU9-NifS | 51.0 | 44.0 | ||
| pN2SB39 | SU9-FdxN-HA | 18.2 | 10.9 | |
| SU9-NifBMi-His10 | 43.8 | 36.4 | ||
Nitrogenase-related proteins and their expected sizes when expressed in yeast from expression vectors generated in this study. Processed forms refer to sizes following predicted SU9 cleavage (RAY-SS, (Vögtle et al., 2009)). See Supplementary Figures S1, S2 for details.