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. 2017 Sep 12;8:1567. doi: 10.3389/fpls.2017.01567

Table 1.

Yeast expression plasmids and yeast strains used in this work.

Strain Transformed plasmids Expressed proteins Full-length (kDa) Processed (kDa)
SB09Y pN2GLT4 SU9-NifU 40.6 33.6
SU9-NifS 51.0 44.0
pN2SB22 SU9-FdxN 17.1 9.8
SU9-NifBAv-His10 63.6 56.3
SB10Y pN2GLT4 SU9-NifU 40.6 33.6
SU9-NifS 51.0 44.0
pN2SB24 SU9-FdxN 17.1 9.8
SU9-NifBMi-His10 43.8 36.4
SB03Y pN2SB15 SU9-FdxN 17.1 9.8
SU9-His10-NifBAv 63.6 56.3
SB12Y pN2GLT4 SU9-NifU 40.6 33.6
SU9-NifS 51.0 44.0
pN2SB39 SU9-FdxN-HA 18.2 10.9
SU9-NifBMi-His10 43.8 36.4

Nitrogenase-related proteins and their expected sizes when expressed in yeast from expression vectors generated in this study. Processed forms refer to sizes following predicted SU9 cleavage (RAY-SS, (Vögtle et al., 2009)). See Supplementary Figures S1, S2 for details.