Figure 3.

FST and dose-response curves of TREK-1 current inhibition by spadin-analogs. (A) Peptides able to inhibit TREK-1 channel activity were tested in the FST. Immobility times were measured 30 min after ip injection and compared with the immobility time obtained with saline injected mice. Spadin was injected at 100 μg/kg and spadin-analogs were injected at 3.2–4.0 μg/kg. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001. (B) Dose-response curves of TREK-1 current inhibition at 0 mV obtained with spadin-analogs compared with spadin. IC₅₀ were 0.1, 0.12, 1.2, and 40.0 nM for PE 22-28, G/A-PE 22-28, biotinylated-G/A-PE 22-28 and spadin, respectively.