Figure 10. Ultrastructural changes induced by Obatoclax.

SCC029B cells when treated with 400 nM Obatoclax for 48 hours induced appearance of numerous autophagic vesicles (Red arrows) containing various cargos. Extensive cytoplasmic vacuolation was apparent with accumulation of electron dense proteinaceous inclusions. Intact mitochondria (those with distinct cristae and internal membranes) were not readily detectable (E–L). Vehicle control cells on the other hand exhibited significantly less cytoplasmic vacuolation and presence of numerous intact mitochondria with distinct cristae (A–D).