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. 2016 Aug 5;8(36):60060–60079. doi: 10.18632/oncotarget.11085

Figure 6. Obatoclax induces necroptosis in OSCC cells.

Figure 6

SCC029B cells were either treated as vehicle control (VC) or exposed to 400 nM Obatoclax (OBT) for 48 hours. (A) The morphology and ultrastructure of control and Obatoclax treated SCC029B cells. (B) A colocalization of p62 with RIP1K and RIP3K in Obatoclax treated SCC029B cells indicate assembly of necrosomal complexes (Scale bar: 10 μm). (C) The Obatoclax treated and vehicle control cell lysates were subjected to coimmunoprecipitation using p62 antibody. IgG: Isotype control antibody. (D) AW8507 and SCC029B cells were treated with 400 nM Obatoclax, 50 μM Necrostatin-1 (Nec-1) or a combination of both for 48 hours and cell viability was quantified by SRB assay. Data is represented as mean ± SEM of three independent experiments. Images are representative of three independent experiments.