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. 2017 May 8;8(36):60109–60122. doi: 10.18632/oncotarget.17686

Figure 8. ERBB1 depletion induces cofilin-1 phosphorylation, which inhibits CDRs formation.

Figure 8

(A) SK-BR-3 wild type (SK-WT) or SK-BR-3 cells transfected with siRNA specific for ERBB1 (SK-siERBB1) were untreated (NT) or treated with Tz for various time points as indicated (in min) and harvested for IP and western blot analysis. Whole cell lysates were analyzed for cofilin phosphorylation (p-cofilin) and total cofilin levels (cofilin). GAPDH was used as loading control. (B) SK-BR-3 cells were transfected with a construct expressing wt cofilin or the S3E cofilin mutant. Histogram bars represent the average ± SD of the percentage of cells with CDRs, observed in 3 replicates. For each experiment at least 3000 cells were analyzed.