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. 2017 Jun 16;8(36):60135–60148. doi: 10.18632/oncotarget.18550

Figure 6. ColXV enhanced adipocyte differentiation and weakened lipolysis via reducing DNA methylation.

Figure 6

(A) Bisulphite sequencing analyses of the ColXV DNA. The methylation sites on promoter and intron 1 of ColXV are depicted. The CpGs in each region are represented with vertical bars. Methylation status of adipocytes was analyzed before (day 0) and after (day 4) differentiation induction (n = 3). Methylation status of the CpGs within each amplified regions were represented with closed circle (methylated) and open circle (unmethylated). (B) Maintenance methyltransferase (mtase) Dnmt1 activity was detected in control, Ad-Col15α1, sh-Col15α1 or 5-Aza-dC treated adipocytes, either on day 0 or day 4 after differentiation (n = 3). 5-Aza-dC or overexpressed and interfered with ColXV treated adipocytes, then relative Col15α1 mRNA level (C) were measured. Relative mRNA levels of C/EBPβ, PPARγ (D) and HSL, ATGL (E) were also detected. Data represent the mean ± SEM of three independent experiments. *#P < 0.05, **##P < 0.01.