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. 2017 Jul 24;8(36):60243–60256. doi: 10.18632/oncotarget.19521

Figure 5. PIM1 expression is repressed by 3′UTR binding of miR-33a.

Figure 5

Relative mRNA level of PIM1 in VCaP cells transfected with either 20 pmol or 60 pmol (A) mimic miR-33a or (B) miR-33a inhibitor. (C) Relative protein level of PIM1 in VCaP cells transfected with either 20 pmol or 60 pmol mimic miR-33a or miR-33a inhibitor. Relative mRNA level of PIM1 in LNCaP cells transfected with either 20 pmol or 60 pmol (D) mimic miR-33a or (E) miR-33a inhibitor. Relative luciferase activity of wild-type and mutated PIM1 3′UTR in LNCaP cells transfected with (F) mimic miR-33a or (G) miR-33a inhibitor. mRNA levels were normalized to β-actin and protein levels were normalized to β-actin, Mean +/− SEM is shown; *P < 0.05, **P < 0.01, ***P < 0.001.