Skip to main content
. 2017 Jul 17;8(36):60312–60323. doi: 10.18632/oncotarget.19294

Figure 6. Western blot and RT-qRCR showed the change in expression of JNK/c-Jun signaling pathway, Sox-9 and COL2.

Figure 6

(A) Western blot was used to evaluate the change in expression of JNK, p-JNK, c-Jun, p-c-Jun, Sox-9 and COL2 protein levels in different groups. (B) Relative levels JNK, c-Jun, Sox-9 and COL2mRNA in the chondrocytes. The 2−ΔΔCt method was adopted with GAPDH as the reference gene. Bars represent the mean ± SD of each group (n = 6). As a control, the membrane was stripped and incubated with β-actin. Significant differences between the groups are marked with asterisks (*P< 0.05, **P< 0.01); ns: no statistical significance.