Table 2. The integrated optical density (IOD) and the positive expression areas of p-JNK, c-Jun, p-c-Jun, Sox-9 and COL2 measured by immunofluorescence.
| NC | IL-1β | HBO | H+I | |||||
|---|---|---|---|---|---|---|---|---|
| IOD | Positiveareas (μm2) | IOD | Positiveareas (μm2) | IOD | Positiveareas (μm2) | IOD | Positiveareas (μm2) | |
| p-JNK | 32.6±9.8 | 22.6±7.2 | 82.3±6.2* | 70.4±8.8* | 38.3±10.4# | 25.6±12.7# | 29.2±7.2# | 20.4±9.2# |
| c-Jun | 30.4±6.8 | 20.3±5.9 | 62.4±8.8* | 52.6±9.8* | 74.3±9.2& | 69.2±10.2& | 66.8±6.2& | 60.8±8.2& |
| p-c-Jun | 26.4±7.2 | 18.9±9.8 | 69.8±10.2* | 58.4±12.7* | 29.4±3.6# | 22.4±5.8# | 29.2±8.2# | 20.5±8.4# |
| Sox-9 | 89.8±8.6 | 67.4±6.2 | 30.2±8.3* | 29.3±9.5* | 86.3±9.2# | 70.4±9.8# | 79.8±10.2# | 66.4±12.2# |
| COL2 | 96.6±5.6 | 79.8±8.8 | 36.1±6.2* | 28.2±6.2* | 76.4±12.6# | 72.3±10.2# | 86.2±6.2# | 79.6±7.4# |
C, control; IL-1β, cells with 10ng/ml interleukin-1β; HBO, hyperbaric oxygen treated cells with 10ng/ml interleukin-1β; H+I, hyperbaric oxygen treated cells with 10ng/ml interleukin-1β and 40μM SP600125 (the JNK inhibitor). *P<0.05, significantly different from the control group; #P<0.05, significantly different from the IL-1β group. &P>0.05, no obviously difference from IL-1β group. Data are represented as the M±SEM of n=8. M, mean; SEM, standard error; n, sample size.