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. 2017 Jul 24;525(15):3227–3250. doi: 10.1002/cne.24273

Figure 3.

Figure 3

Gpr151 mRNA expression in Gpr151‐Cre neurons. Coronal section of a Gpr151‐Cre mouse injected with AAV8‐hSyn1‐FLEX‐mCherry in the habenular region (−1.82 mm posterior to Bregma). Gpr151 mRNA expression (green) can be seen in the ventral medial habenula, lateral habenula (excluding the oval subnucleus of the lateral division), as well as in the lateral posterior, central lateral, and paraventricular thalamic nuclei (panels a, c, d, e). Expression of mCherry (magenta, panels b–e) show partial overlap with Gpr151 mRNA. Transduction efficiency and injection localization could explain the lack of mCherry expression in certain Gpr151‐positive subregions like the LHbMS. Examples of coexpression of mCherry and Gpr151 mRNA in the lateral posterior thalamic nucleus and lateral habenula are shown in panel (d) and (e) (magnifications of insets in panel c). Scale bar (a–c) = 100 µm, (d, e) = 20 µm