Figure 6.

TNF‐α down‐regulated osteoclast‐related genes and up‐regulated TNF‐α receptors in monocyte cultures on plastic. Fold increase (compared to day 0) of genes (a) TRAcP, (b) NFATc1, (c) RANK, (d) TNF‐α R1, and (e) TNF‐α R2, of monocyte cultures on plastic. Cells were cultured on plastic either with 30 ng/ml M‐CSF and 20 ng/ml RANKL (white column) or with 30 ng/ml M‐CSF, 20 ng/ml RANKL and 10 ng/ml TNF‐α (black column). The cultures were stopped at day 3 or day 5. (n = 6, *p < 0.05, **p < 0.01, ***p < 0.001)