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564Igi AicdaG23S and 564Igi mice have equal numbers of splenic and bone marrow (BM) B cells. Total BM (A–C) or spleen (D) cell suspensions from the indicated mice were stained with fluorescent antibodies and analyzed by flow cytometry to detect B220+ B cell (A,D) B220+ IgM− pre B cells (B) and B220+ IgM+ immature B cells (C). Shown is the absolute number of live cells in each animal with the mean shown as a horizontal line. Each point represents an individual animal.
