Figure 5.
Calorimetric data of apoA-I–DMPC samples differing in protein:lipid ratios. ApoA-I:DMPC molar ratios are indicated. DSC (A) and PPC (B) data were recorded during heating from 5 to 100 °C at rates of 90 and 80 °C/h, respectively. Fresh rHDL samples containing 0.5 mg/mL protein in 10 mM sodium phosphate buffer (pH 7.5) were used in each experiment. The chain melting phase transition at Tc, lipoprotein denaturation at Tm,app, and unfolding of lipid-free and lipid-poor apoA-I at Tm are indicated.