Acinetobacter baumannii biofilm treated with antibiotics alone or in combination with maltodextrin (MD, 20 mM) (A), sucrose (80 mM) (B), 3,350-Da PEG (6.6 mM) (C), or 400-Da PEG (26.6 mM). Antibiotics included tobramycin (Tob; 50 μg/ml), chloramphenicol (Chlor; 300 μg/ml), ciprofloxacin (Cip; 20 μg/ml), nalidixic acid (Nal; 30 μg/ml), and erythromycin (Ery; 100 μg/ml). Error bars represent the standard errors of the means calculated from three biological replicates. Statistical significance was calculated using one-way ANOVA with paired comparisons (Tukey's test) for individual antibiotics with or without osmotic agent (*, P < 0.05; ns, not significant).