TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| A. caulinodans | ||
| ORS571 | WT | 1 |
| Anx3 | Δkup | This study |
| Anx101 | ΔkdpA | This study |
| Anx104 | ΔtrkA ΔtrkI ΔtrkJ | This study |
| Anx105 | Δkup ΔtrkJ | This study |
| Anx106 | ΔkdpA ΔtrkA ΔtrkI ΔtrkJ | This study |
| Anx107 | Δkup ΔtrkA ΔtrkI ΔtrkJ | This study |
| Anx108 | Δkup ΔkdpA | This study |
| Anx191 | Δkup ΔtrkA | This study |
| Anx192 | Δkup ΔtrkI | This study |
| Anx193 | Δkup ΔtrkA ΔtrkI | This study |
| Anx194 | Δkup ΔtrkA ΔtrkJ | This study |
| Anx195 | Δkup ΔtrkI ΔtrkJ | This study |
| Anx112 | Δkup ΔkdpA ΔtrkJ | This study |
| Anx124 | WT kup-lacZ | This study |
| Anx138 | WT kdpF-lacZ | This study |
| Anx126 | WT trkA-lacZ | This study |
| Anx127 | WT trkJ-lacZ | This study |
| Anx139 | Δkup kdpF-lacZ | This study |
| Anx197 | Δkup ΔtrkA kdpF-lacZ | This study |
| Anx198 | Δkup ΔtrkI kdpF-lacZ | This study |
| Anx199 | Δkup ΔtrkA ΔtrkI kdpF-lacZ | This study |
| Anx200 | Δkup ΔtrkJ kdpF-lacZ | This study |
| Anx201 | Δkup ΔtrkA ΔtrkJ kdpF-lacZ | This study |
| Anx202 | Δkup ΔtrkI ΔtrkJ kdpF-lacZ | This study |
| Anx141 | Δkup ΔtrkA ΔtrkI ΔtrkJ kdpF-lacZ | This study |
| Anx114 | ΔtrkJ kdpF-lacZ | This study |
| E. coli | ||
| DH5α | F− ϕ80ΔlacZΔM15 Δ(lacZYA-argF)U169 recA endA hsdR supE44 thi gyrA relA1 | 36 |
| S17-1(λpir) | F− ompT hsdSB(rB− mB−) gal dcm(DE3) Δ(srl-recA)306::Tn10 (Tetr) | 29 |
| Plasmids | ||
| pK18mobsacB | Suicide vector for gene disruption | 28 |
| pTA-MTL | Suicide vector for lacZ fusion | 30 |
| pTAC3 | pK18mobsacB carrying kup deletion fragment | This study |
| pTAC39 | pK18mobsacB carrying kdpA deletion fragment | This study |
| pTAC110 | pK18mobsacB carrying trkA deletion fragment | This study |
| pTAC111 | pK18mobsacB carrying trkI deletion fragment | This study |
| pTAC40 | pK18mobsacB carrying trkAI deletion fragment | This study |
| pTAC41 | pK18mobsacB carrying trkJ deletion fragment | This study |
| pTAC54 | pTA-MTL carrying kup-lacZ fusion | This study |
| pTAC58 | pTA-MTL carrying kdpF-lacZ fusion | This study |
| pTAC56 | pTA-MTL carrying trkA-lacZ fusion | This study |
| pTAC57 | pTA-MTL carrying trkJ-lacZ fusion | This study |