TABLE 2.
Primers used in this study
| Primer | Sequence | Description of underlined sequence |
|---|---|---|
| Plasmid construction | ||
| pTAC3 | ||
| Acp16 | CGAATTCTGATCCGGCACATTGTCA | EcoRI |
| Acp17 | GTCGAGATTGGTCACCCGCCAGGTGATGAGCGACAG | Complement with Acp18 |
| Acp18 | CGGGTGACCAATCTCGAC | |
| Acp19 | GCGGATCCGGATGGGATGACGCTAC | BamHI |
| pTAC39 | ||
| Acp254 | AACTGCAGCCTGATCGGGATTGAACG | PstI |
| Acp192 | ACGATGTAGGCGAAGCGGAGCGTGTAGCCGATCCAG | Complement with Acp193 |
| Acp193 | CCGCTTCGCCTACATCGT | |
| Acp255 | GCTCTAGAGAGGGCGGAGATCTTGGT | XbaI |
| pTAC110 | ||
| Acp198 | AACTGCAGAGCGCCTGCTGATTCTC | PstI |
| Acp579 | GCAGGCCACCATGTTCAC | |
| Acp584 | GTGAACATGGTGGCCTGCGTCAGCCTCGAATTCTTCTGA | Complement with Acp579 |
| Acp585 | GCTCTAGATGGCTTCCACCGTCTC | XbaI |
| pTAC111 | ||
| Acp586 | AACTGCAGTCAGCCTCGAATTCTTCTGA | PstI |
| Acp587 | CAGGATCAGGCTCTCGCCGATGGCTTCCACCGTCTC | Complement with Acp207 |
| Acp207 | GGCGAGAGCCTGATCCTG | |
| Acp208 | GCTCTAGAGGCGAGGTGGATGATGAC | XbaI |
| pTAC40 | ||
| Acp198 | AACTGCAGAGCGCCTGCTGATTCTC | PstI |
| Acp253 | CAGGATCAGGCTCTCGCCGCAGGCCACCATGTTCAC | Complement with Acp207 |
| Acp207 | GGCGAGAGCCTGATCCTG | |
| Acp208 | GCTCTAGAGGCGAGGTGGATGATGAC | XbaI |
| pTAC41 | ||
| Acp212 | CGAATTCGGCCATGTGGAAGAGGTG | EcoRI |
| Acp213 | AACAGACGCACTTCCGGAATGTTCCGCCACAAGAGC | Complement with Acp214 |
| Acp214 | TCCGGAAGTGCGTCTGTT | |
| Acp215 | GCGGATCCCGTCATCCGCATATCGT | BamHI |
| pTAC54 | ||
| Acp256 | GCGGATCCACAAGAAGCGCATCATCCTT | BamHI |
| Acp285 | AACTGCAGAAGATACCAGCGCAGACC | PstI |
| pTAC58 | ||
| Acp307 | AACTGCAGCAAGTCCATCTCCTCCTCCA | PstI |
| Acp308 | GCTCTAGAATCATCACGCGCTCCTTC | XbaI |
| pTAC56 | ||
| Acp198 | AACTGCAGAGCGCCTGCTGATTCTC | PstI |
| Acp287 | GCTCTAGAGCAGGCCACCATGTTCAC | XbaI |
| pTAC57 | ||
| Acp212 | CGAATTCGGCCATGTGGAAGAGGTG | EcoRI |
| Acp288 | GCGGATCCATGTTCCGCCACAAGAGC | BamHI |
| RT-PCR | ||
| kup | ||
| P1 | AGATCGAGCTCTGGACGAAG | |
| P2 | TATTTGACGGTGACGGAAAG | |
| P3 | CACAGCAACCGTGAACGAC | |
| P4 | TTCACCGGATAACCGATCTC | |
| P5 | CGGGTGACCAATCTCGAC | |
| P6 | ATAAATCCCAAGCCATGCAG | |
| kdpFABC | ||
| P7 | GACCGAGATCTCCACCAAGA | |
| P8 | GCAGGATGTAGAGGGTCGAA | |
| P9 | CTCGCCGCTTATCTCCTCAC | |
| P10 | GAGATCACGGATGAACAGCA | |
| P11 | CCGCTTCGCCTACATCGT | |
| P12 | AGGAATTGGCGGCATCATAG | |
| P13 | GCCATCACCCTTCTGGTG | |
| P14 | ATGTCGAATTCAGGGACGAG | |
| trkAI | ||
| P15 | TCGACCTCATCTCTCAGACG | |
| P16 | GATTGCTCTCCACCACCTTG | |
| P17 | AGAACGACGTCTCCATCGTC | |
| P18 | GGCGAGGTGGATGATGAC | |
| P19 | GGCGAGAGCCTGATCCTG | |
| P20 | GAGGTGGATGATGACGAAGG | |
| trkJ | ||
| P21 | TCTGGGCCTATACGATCGAG | |
| P22 | TGAAGACGATGGCCACATAG | |
| P23 | GCTGTTCGAGAGCATGTCC | |
| P24 | GCTCGCCACATAAAGGAAGA | |
| P25 | TCCGGAAGTGCGTCTGTT | |
| P26 | GCGAAGAGCAGAAGGAGATG |