FIG 8.

Planktonic cellular aggregate development and biofilm formation are dependent on LdhR and LdhA activities. (A) Images of planktonic aggregates formed during growth of B. multivorans ATCC 17616 wild-type cells and the ΔldhR deletion mutant in medium supplemented with 2% d-mannitol for the indicated times. (B) Images of the planktonic cellular aggregates that sedimented after 5 min of static incubation of the indicated cultures grown for 72 h under constant agitation in medium containing 2% d-mannitol. (C) Images of biofilm cells attached to the solid surface of 24-well microtiter plates after 24 h of incubation with agitation in medium containing 2% d-mannitol, and biofilm quantification by the crystal violet staining method. Bars, 2.5 mm.