TABLE 2.
Pimelic acid production by strain WTI-1 with amplified fatty acid biosynthesis genesa
| Strain (plasmid) | Growth (OD660) | Pimelic acid (μg · liter−1) |
|---|---|---|
| ATCC 13032 | 47.5 ± 1.5 | —b |
| WTI-1 (vector) | 47.1 ± 1.7 | 20.3 ± 0.7 |
| WTI-1 (pCaccBCDE) | 46.3 ± 2.1 | 46.7 ± 2.3 |
| WTI-1 (pCfasA) | 46.5 ± 2.0 | 61.3 ± 2.9 |
| WTI-1 (pCfasB) | 46.1 ± 2.2 | 35.9 ± 1.2 |
a
Production was carried out in fermentation medium LFG1 containing 5% glucose in 300-ml baffled Erlenmeyer flasks. After glucose was consumed, the culture supernatants were subject to LC-MS/MS analysis to determine the amounts of pimelic acid. The detection limit of pimeric acid is approximately 1.0 μg · liter−1 under our analytical conditions. Values are means and standard deviations of the results from three independent experiments.
b
—, not detected.