Effects of bacillomycin D on deoxynivalenol (DON) biosynthesis (A) and the expression of DON-related genes (B) of F. graminearum. Data are expressed as means ± SDs. *, significant difference, compared with controls (P < 0.01). In the quantitative real-time PCR analysis, values were normalized to the levels of the actin gene, as an internal reference; gene expression values are reported as means ± SDs, relative to control values. The experiments were repeated independently three times.