Figure 1.

(A) Representative competition association assay curves of [³H]34 in the absence (control) or presence of a long residence time compound 27 and a short residence time compound 5. Experiments were performed at 10 °C using the compound’s respective IC₅₀ value at the hA₃R. (B) Competition association curves of [³H]34 in the absence (control) or presence of long residence time compound 27. Experiments were performed at 25 °C using the compound’s respective IC₅₀ value at the hA₃R. t₁ is the radioligand binding at 20 min, while t₂ is the radioligand binding at 240 min.