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. 2017 Aug 10;8(9):963–968. doi: 10.1021/acsmedchemlett.7b00280

Figure 4.

Figure 4

NADPH-coupled GPx assay for compounds 10, SLC-0111, and 22. Reaction conditions: [NADPH]0 = 0.6 mM, [GSH]0 = 2.0 mM, [H2O2]0 = 5 mM, [GR] = 8 units/mL, [catalyst] = 0.2 mM in pH 7.4 phosphate buffer at room temperature. Reported are the mean ± SD values of three separate experiments.