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. 2017 Sep 18;3:17063. doi: 10.1038/cddiscovery.2017.63

Figure 3.

Figure 3

ROS generation by lauric acid is involved in the activation of transduction signaling observed in breast and endometrial cancer cells. (a) ROS production determined as DCF fluorescence in SkBr3 and Ishikawa cells treated for 60 min with vehicle (−) or 100 μM LA alone or in combination with 300 μM free radical scavenger NAC. DCF fluorescence obtained in cells treated with vehicle was set as onefold induction upon which ROS levels induced by treatments were calculated. Data shown are the mean±S.D. of three independent experiments performed in triplicate. (○) indicates P<0.05 for cells receiving vehicle versus treatments. EGFR, ERK1/2 and c-Jun activation in SkBr3 (b) and Ishikawa (c) cells treated for 60 min with vehicle or 100 μM LA alone or in combination with 300 μM NAC. EGFR, ERK2 and c-Jun were used as loading controls for pEGFR, pERK1/2 and pc-Jun, respectively. Results shown are representative of at least two independent experiments.