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. 2017 Sep 18;3:17063. doi: 10.1038/cddiscovery.2017.63

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Lauric acid regulates the expression of cell cycle regulatory genes. The mRNA expression of c-fos, p53 and p21^Cip1/WAF1 (p21) was evaluated by real-time PCR in SkBr3 (a) and Ishikawa (b) cells treated with vehicle (−) or 100 μM LA, as indicated. Data obtained from three independent experiments performed in triplicate were normalized to 18 S expression and shown as fold changes of mRNA expression induced by LA respect to cells treated with vehicle. Evaluation of c-fos, AP1 and p21 luciferase reporter genes in SkBr3 (c) and Ishikawa (d) cells treated for 18 h with vehicle or 100 μM LA. The luciferase activities were normalized to the internal transfection control, and values of cells receiving vehicle were set as onefold induction upon which the activity induced by treatments was calculated. Data shown are the mean±S.D. of three independent experiments performed in triplicate. (○) indicates P<0.05 for cells receiving vehicle versus treatments. c-fos, p53 and p21 protein levels in SkBr3 (e) and Ishikawa (f) cells treated with vehicle or 100 μM LA, as indicated. β-actin was used as a loading control. Results shown are representative of at least two independent experiments.