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. 2017 Sep 18;3:17063. doi: 10.1038/cddiscovery.2017.63

Figure 7.

Figure 7

Lauric acid induces apoptotic cell death. (a, d) TdT-mediated dUTP nick-end-labeling (TUNEL) staining (green) in SkBr3 (a) and Ishikawa (d) cells treated for 18 h with vehicle (−) or 100 μM LA alone or in combination with 300 μM free radical scavenger NAC and 10 μM p21Cip1/WAF1 inhibitor UC2288, as indicated. Nuclei were stained by propidium iodide (PI, red). Magnification is indicated by bars (100 μm). Each experiment shown is representative of 20 random fields observed. (b, e) Bar graphs represent the percentage of TUNEL-positive cells upon treatments versus vehicle. Values are the mean of three independent experiments. (○) indicates P<0.05 for cells receiving vehicle versus treatments. (c, f) Efficacy of p21Cip1/WAF1 downregulation by UC2288. β-actin was used as a loading control. Results shown are representative of at least two independent experiments.