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. 2017 Sep 13;8:642. doi: 10.3389/fphar.2017.00642

FIGURE 4.

FIGURE 4

Lack of H4R expression enhances protein expression of inflammation-associated cytokines and chemokines. Wild type (WT) or H4R-deficient (H4R-/-) BALB/cJ mice were treated with 2 mg/100 μlmouse TNBS (TNBS) or with an equivalent volume of the solvent mixture EtOH/PBS (EtOH). After dissection, the cola were cut in three sections, proximal, medial, and distal. From parts of the medial sections proteins were extracted and analyzed by Luminex Array (cytokines, chemokines) and ELISA (MPO). Analyte concentrations are reported relative to the total protein concentrations of the colon lysates. ∗∗∗p < 0.005 (One-Way ANOVA), TNBS: n = 7/group; EtOH: n = 4/group.