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. 2017 Sep 13;8:474. doi: 10.3389/fneur.2017.00474

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Copyright © 2017 Jin, Han, Zhang, Zhang, Li, Feng, Qin, Kong, Nie, Li and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Separation and identification of glioma stem cells. (A) The TJ905 cells were cultured in serum-free medium. The cells grew closely with few pseudopodia. (B) The TJ905 stem cells grew together to spheres in suspension after 3–4 days. (C) Immunofluorescence staining showed that the nestin immunostaining for spheres was strongly positive. (D) After culture in medium containing 10% fetal bovine serum, the spheres differentiated, appearing with many pseudopodia and showing many kinds of irregular shapes, such as triangle, round, and star shapes. (E) The GFAP immunostaining for differentiated glioma stem cells was strongly positive and nestin staining was negative. (F) The β-tubulin immunostaining for differentiated glioma stem cells was strongly positive.