FIGURE 3.

Relative expression of T3Es genes with FA treatment by qRT-PCR. The OE1-1 was grown in hrp-inducing medium (with 0.5 mM of FA or DMSO control) to OD₆₀₀ as about 0.1 and total RNA was isolated for qRT-PCR analysis. Eight representative T3Es genes for selected for determination and the serC gene was used as reference gene for normalization of gene expression. Normalized value with FA treatment was divided with that of control and relative values were presented. Each test was repeated from RNA isolation in at least three independent trials and each trials included four replications. Mean values were averaged and presented with SD (error bars). Statistical significance between FA treatment and the DMSO control was assessed using a post hoc Dunnett test following ANOVA. Significance level, ^∗p < 0.05 and ^∗∗p < 0.01.