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. 2017 Sep 15;8:545. doi: 10.1038/s41467-017-00515-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Analysis of HIV-1 lipid membrane properties by C-Laurdan staining and after cholesterol depletion. a Median values of the generalised polarisation (GP) parameters were determined from pixels of spectral images corresponding to C-Laurdan and mCherry.Vpr labelled mature and immature virus particles. Results represent measurements from 40 particles each from two independent virus preparations and are shown in a box (IQR) and whisker (min/max) plot. The statistical significance was assessed using Wilcoxon rank-sum test. b Median Env diffusion coefficient (D) of cholesterol oxidase (COase) treated HIV-1 was determined by sSTED-FCS measurements of 50 mature particles each from two independent virus preparations. Results are shown in a box (IQR) and whisker (min/max) plot and the statistical significance was assessed by Wilcoxon rank-sum test