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. 2017 Sep 15;7:11725. doi: 10.1038/s41598-017-12010-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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UV laser induces DNA-protein binding. (a) Western blotting analysis on chromatin derived from formaldehyde- and UV laser-treated cells. Histone extract was used as internal control. (b) Venn diagram showing number of proteins identified by MS/MS analysis within chromatin complexes derived from formaldehyde (red)- and laser (blue)-treated cells. Rectangles contain GO term analyses of each protein (formaldehyde = red, UV laser = blue and common proteins = violet). (c and d) ChIP assays performed in indicated cells treated with E2 and crosslinked with formaldehyde and UV laser showing TFF1 promoter region occupancy by (c and d, top) ERα and (d, bottom) GFP. (e,f) ChIP assays performed in indicated cells treated with MS-275 at indicated times and crosslinked with formaldehyde or UV laser showing TRAIL promoter region occupancy by H3K9K14ac (e,f, top) and H3K4me3 (e and f, bottom). All ChIP data obtained on immunoprecipitated fractions were normalized to input chromatin (IP/Input). Curves show the mean of at least two independent experiments with error bars indicating standard deviation.