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. 2017 Sep 15;7:11696. doi: 10.1038/s41598-017-12064-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of proteinuria and kidney histology. (a) Representative images of H&E staining of WT, Col4a3+/−, and Col4a3−/− kidney sections. Glomeruli in wild-type (WT) and Col4a3+/− kidneys have a normal appearance. The right upper panel shows several sclerosed glomeruli (arrows) along the surface of a cortical lesion and interstitial injury in a Col4a3−/− kidney. Scale bars, 50 μm in upper panels; 10 μm in lower panels. (b) Coomassie blue staining reveals a strong albumin band in spot urines collected from Col4a3−/− mice but not from the WT and Col4a3+/− mice.