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. 2017 Aug 18;31(9):1308–1316. doi: 10.1038/eye.2017.158

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Subretinal gene therapy injection system. (a) A dual-bore 41 G blunt polytetrafluoroethylene (Teflon) tipped cannula mounted within a 23 G steel shaft (DORC, Zuidland, The Netherlands). Arrows indicate proximal and distal pressure relief holes along the shaft, which allow evacuation of excess vitreous fluid out of the eye during the injection. (b) Components of the subretinal injection system consisting of a 1 ml BD syringe, custom syringe lock with a rubber O-ring, air filter and polyethylene connection tubing (Alchimia, Ponte S Nicolò, Italy). (c) Assembled injection system.