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. 2017 Sep 15;7:11674. doi: 10.1038/s41598-017-12021-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Graphical representation of differentially expressed/abundant proteins. Before normalization, the data revealed some variation between individuals (A–yellow bars). However, after the quantile-normalization procedure (A–green bars), the mean value and standard error bars show almost no variation between the samples. Significant differentially-expressed proteins are demonstrated with MA plots and are more common in estrus than in proestrus or metestrus (B,C). PLGEM model was involved in testing the differences in normalized signal values between proestrus and estrus (B), and between metestrus and estrus (C). The level of significance (PvsE, MvsE) is scaled from green (P < 0.05) to blue (P < 0.01) and only the data points with FC > 2 are annotated. The x-axis represent the basal mean of signal intensities in B and C. The dependence of particular fold changes on p-values is provided using the volcano plots in D and E.