Table 3.
Oligonucleotide sequences for primers used in dsRNA construct design. Underlined sequences are the T7 promoter sequences.
Target genes | Forward primer | Reverse primer |
---|---|---|
SgVKR1 | 5′-TAATACGACTCACTATAGGGAGAAAGGGTTGCTGCATTGACTG-3′ | 5′-TAATACGACTCACTATAGGGAGATCCACACTGCATCATAGGCA-3′ |
SgVKR2 | 5′-GAAATTAATACGACTCACTATAGGGCCCACTGTGTATGGAGGAGAA-3′ | 5′-GAAATTAATACGACTCACTATAGGGCCTCAAACATTGGCCTTGTC-3′ |
GFP | 5′-TAATACGACTCACTATAGGGAGA AAGGTGATGCTACATACGGAA-3′ | 5′-TAATACGACTCACTATAGGGAGA ATCCCAGCAGCAGTTACAAAC-3′ |
Abbreviations: VKR = venus kinase receptor, GFP = green fluorescent protein.