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. 2017 Jun 15;8(35):57938–57947. doi: 10.18632/oncotarget.18488

Figure 3. Cell cycle arrest at G1 phase by MELK knockdown.

Figure 3

A. Silencing of MELK by siRNA reduced the number of viable cells on both HCT116-p53(+/+) and -p53(-/-) cells. The asterisk indicates p < 0.01 compared with the corresponding value of the siControl group. B. Cell cycle analysis was performed in HCT116-p53(+/+) and -p53(-/-) cells treated with siControl and siMELK for 48 hours. Bromodeoxyuridin (Brdu) staining and 7-aminoactinomycin D (7AAD) were used to analyze the cell cycle. C. FACS analysis of HCT116-p53(+/+) and -p53(-/-) cells treated with siControl and siMELK for 48 hours. Cells were collected at 0, 3, and 6 hours after the release of cell cycle arrest caused by aphidicolin.